SLC16A3 and central nervous system cancer: To investigate the functional consequences of MCT4 expression in glioma, we generated an MCT4 overexpression and knockdown/inhibition model in F98 glioma cells, which were also shown to upregulate MCT4 under hypoxia, and performed several in vitro cell culture assays as well as an ex vivo slice culture model, much closer reflecting the in vivo situation.