In addition, we collected heart tissue from DCM patients and control subjects, and analyzed the expression of hub lncRNAs in this local cohort using quantitative RT-PCR, which showed elevated expression of six hub lncRNAs (FGD5-AS1, AC009113.1, WDFY3-AS2, NIFK-AS1, MIR100HG, and ZNF571-AS1) in heart tissue from DCM patients relative to that in tissue from control subjects (Figure 5F). This evidence concerns the gene MIR100HG and familial dilated cardiomyopathy.