ACTA1 and diabetes mellitus: In addition, we detected the expression of α-SMA in bladder tissue by WB and immunofluorescence staining and found that the expression of α-SMA was decreased in the DBD group (vs. Control p < 0.05) and increased in the DBD + IR-61 group (vs. DBD group p < 0.05), which also indicated that IR-61 could prevent the decreased smooth muscle content caused by DM.