The use of neutralizing antibody is a viable approach to elucidate the beneficial role of a protein of interest after blocking its function, as seen in a study where IL-10 reversed the Dinitrobenzene-sulfonic acid-induced colitis in mice, and this anti-inflammatory effect was blocked by treatment with neutralizing IL-10 antibody (Hunter et al., 2005). The gene discussed is IL10; the disease is colitis.