As few as 1 × 103 of lnc408-overexpressing BT549 cells was sufficient for tumor initiation (2/10 vs 0/10), 1 × 104 of lnc408-overexpressing BT549 cells led to more tumor initiation (4/10 vs 1/10), and injection of 1 × 105 of lnc408-overexpressing BT549 cells resulted in tumorigenesis in most of mice in comparison of non-BCSC cells (8/10 vs 2/10; Fig. 3E), suggesting that lnc408 can enable non-CSCs to acquire stemness characteristic in breast cancer. Here, TOMM22-DT is linked to breast cancer.