Bivariate statistical analysis was performed between the gene expression ratios observed by real-time RT-PCR on the six selected genes (including GAPDH) in the cohort of 12 subjects (6 subjects with DS and 6 normal controls) and the expression ratios of the same genes in the original group of 8 subjects (4 subjects with DS and 4 normal controls) whose samples had also been subjected to RNA-Seq as described above (r=0.88, p=0.0186) (Table 2B). This evidence concerns the gene GAPDH and Dravet syndrome.