Finally, using Toll-like receptor 3 (TLR3) knockdown NK cells,chemical blockade of TLR3/double-stranded RNA (dsRNA) complex,and chemical inhibition of the TLR3 downstream pathway, we further determined that reovirus activation of NK cells is mediated in a TLR3 signaling pathway-dependent manner.These findings of synergistically enhanced NK cell anti-tumor activity provide a viable framework for improvement of clinical strategies against CRC. This evidence concerns the gene TLR3 and colorectal carcinoma.