To understand the role of MICU2 in stimulus-secretion coupling in pancreatic β cells, we silenced MICU2 in INS-1 832/13 cells, a rodent-derived insulinoma cell line [20], and in EndoC-βH1 cells, a human embryonic insulin-secreting cell line [21,30]. Here, MICU2 is linked to pancreatic insulinoma.