Subgroup analysis of OS showed that specific subgroups may benefit most from LH, such as older than 50 years, female sex, tumor located in the adjacent two segments, multiple tumors, maximum tumor size larger than 10 cm, presence of microvascular invasion, preoperative AFP ≥400 ng/ml, HBV DNA >1000 IU/ml, and margin width ≤2 cm (Figure 4). This evidence concerns the gene PLOD1 and neoplasm.