Intriguingly, confocal imaging showed that the GFP‐LC3 puncta generated by PD treatment were not colocalized with LysoTracker Red in both GBM cells, which were similar to those of cells treated with BafA1 that blocks lysosomal fusion with autophagosomes by inhibiting vacuolar H+ ATPase‐dependent lysosomal acidification [21] (Fig. 2A,B). This evidence concerns the gene MAP1LC3A and glioblastoma.