By quantification of the MAP2- and NF-L-related immunofluorescence intensities (Fig. 2) in the applied mouse models, the reduction of MAP2-related immunofluorescence intensity in stroke tissue was found to be significant after 4h-p, 24h-t, 24h-p, and 72h-t compared to control regions. The gene discussed is NEFL; the disease is Stroke.