The association between HBV and SIRT4 was also observed in the HCC cell lines studied, in which the HCC cell lines harboring HBV (HepG2.2.15, SNU-387, and Hep3B) tended to have a lower expression of SIRT4 than those without HBV (HepG2 and Huh7) (Fig. 3A). This evidence concerns the gene SIRT4 and hepatocellular carcinoma.