To investigate the role of POU2F2-PDPK1 axis in GBM tumorigenesis, we injected LN229 shCtrl, LN229 shPOU2F2, and LN229 shPOU2F2 cells with reconstituted expression of PDPK1 intracranially into nude mice. This evidence concerns the gene POU2F2 and glioblastoma.