Taken together, all of our results demonstrated that POU2F2 directly promoted the transcription of PDPK1, and then POU2F2 interacted with AKT, activated PI3K/AKT/mTOR pathway to increase the expression of GLUT1, HK2, and PKM2, leaded metabolic shift towards aerobic glycolysis, and promoted GBM progression (Fig. 8). This evidence concerns the gene MTOR and glioblastoma.