To investigate the role of POU2F2-PDPK1 axis in GBM tumorigenesis, we injected LN229 shCtrl, LN229 shPOU2F2, and LN229 shPOU2F2 cells with reconstituted expression of PDPK1 intracranially into nude mice. The gene discussed is PDPK1; the disease is glioblastoma.