We found that GRHL1 promoted the proliferation of NSCLC by regulating the transcription of G2/M phase-related genes: cell division cycle 27 (CDC27), anaphase promoting complex subunit 13 (ANAPC13 or APC13), RAD21 cohesive complex (RAD21) and cell division cycle 7 (CDC7). This evidence concerns the gene GRHL1 and non-small cell lung carcinoma.