To validate the RNA-Seq data, expression of CYP2B7P, CYP2F2P and CYP4F26P that were involved in arachidonic acid metabolism, as well as IGHV3–69-1 and FCGR1CP participating in Fc gamma related immune system were detected by qRT-PCR in 10 pairs of NPCs and their paracancerous samples, as well as in NPC cell lines (HNE1, CNE1 and CNE2) and NP69 normal human nasopharyngeal epithelial cell line. The gene discussed is CYP4F26P; the disease is nasopharyngeal carcinoma.