To assess the consequences of the two rare CDH1 germline variants, c.1774G > A p.(A592T) and c.2450C > T p.(A817V), identified in glioma families 1 and 2, we performed functional analyses using two cellular models, i.e. CRISPR/Cas9-mediated HEK293T knock-in and stably transfected CHO cells. This evidence concerns the gene CDH1 and glioma.