In order to explore the role of NRT in patients with NSCLC.DCs from P03 (HLA-A*02:01+) loaded with ACAD8-T105I, BCAR1-G23V and PLCG1-M425L were cocultured with PBLs to generate mutated peptide-specific NRTs in vitro, and the immune responses were compared with the WT peptide-induced NRTs. Here, BCAR1 is linked to non-small cell lung carcinoma.