GPC1 and Familial prostate cancer: GPC-1 inhibition in HS-5 cells increased gap closure after exposure to CCM from either DU-145 or PC-3 cells after 48 h, as compared to control HS-5 cells (Fig. 4D–G), indicating that GPC-1 inhibition in HS-5 cells increased their migration when exposed to prostate cancer CCM.