Moreover, motif analysis with Hypergeometric Optimization of Motif EnRichment (HOMER) [47] identified that gain-of-5hmC was enriched at over 40% of target sequences with Nanog and Scl (stem cell leukemia) motifs (FDR < 0.05, Fig. 2d and Additional file 6: Table S5). Here, NANOG is linked to acute leukemia.