We used the TIMER algorithm to estimate the abundances of six immune infiltrates (B cells, CD4 + T cells, CD8 + T cells, Neutrophils, Macrophages, and Dendritic cells) in GC samples, and found that the infiltration level of CD8 T cells, dendritic cells, neutrophils and Macrophages exhibited obviously difference in the mutated-TP53 and WT-TP53 GC tissues (Fig. 1a). This evidence concerns the gene CD4 and gastric cancer.