To better understand why some individuals with obesity develop CVDs while others remain disease-free, methylation levels of several mtDNA genes, including MT-CO1, MT-CO2, MT-CO3, MT-TL1 and MT-TF, and of the D-loop and light-strand origin of replication (MT-OLR) regions were evaluated in platelet mtDNA from 200 adults with overweight and obesity, of whom 84 developed cardiovascular disease [83]. Here, TF is linked to obesity due to melanocortin 4 receptor deficiency.