First, to clarify the role of purinergic receptors in RT-R-BC cells, we observed the mRNA expression levels of purinergic receptors, including P2Y2R and ADO receptors, in various BC cells (MDA-MB-231, MCF-7, T47D) and RT-R-BC cells (RT-R-MDA-MB-231, RT-R-MCF-7, RT-R-T47D), with the normal epithelial cell line MCF-10A as the control. The gene discussed is P2RY2; the disease is breast cancer.