Via Real-Time PCR, we validated the expression of 3 genes with increased expression in males (TTTY15, UTY, and KDM5D, Figure 1d) and 2 genes with decreased expression (XIST, ROR2, Figure 1e) in an independent cohort of SAT samples obtained from 11 females affected by obesity and 7 males affected by obesity, supporting the evidence of the RNA-seq analysis. This evidence concerns the gene KDM5D and obesity disorder.