Each of these three genes harbor variants previously associated with hearing loss in various syndromes (Table S1): branchio-otic syndrome with ossicular chain anomalies for ANLN [6]; Weiss-Kruszka syndrome with craniofacial dysmorphisms and developmental delay for ZNF462 [51]; and Joubert syndrome with cerebral, retinal and kidney disease for CEP290 [52]. This evidence concerns the gene ANLN and Global developmental delay.