The cut-off value for IL-6 that best distinguishes OSCC patients from controls was 15.038 pg/mL (81.82% sensitivity and 96% specificity), for IL-8 was 923.78 pg/mL (75.76%sensitivity and92% specificity), for TNF-α was 17.01 pg/mL (86.36% sensitivity and 100% specificity), and for HCC-1 was 112.90 pg/mL (83.33% sensitivity and 92% specificity) selected by the Youden index. The gene discussed is IL6; the disease is hepatocellular carcinoma.