We injected SCID/Beige mice intramuscularly with MuLE lentiviruses expressing either shRNA-Cdkn2a + HrasG12V, shRNA-Trp53 + HrasG12V or shRNA-Trp53 + shRNA-Pten + HrasG12V and monitored tumor growth with BLI, sacrificing mice on an individual basis once the primary tumors reached approximately 109 p/s in BLI. Here, CDKN2A is linked to neoplasm.