To identify possible mechanisms and ways of preventing mineral bone disorders, we aimed to compared the mineralization competence of two human osteoblast-like cell lines: hFOB 1.19 osteoblasts (close to normal osteoblasts) and Saos-2 osteosarcoma (close to skeletal cancer) and to correlate the different examined intracellular distribution of AnxA2, AnxA6, FetuA, and TNAP in these cellular models with their mineralization competence. Here, ANXA6 is linked to osteosarcoma.