Although ovx females on HFD have lower lipid accumulation, depletion of either ovary hormones or both ovary hormones and Sirt3 changes the expression of cyp2e1 and cyp4a14. Higher expression of either of these genes indicates that these mice are prone to NAFLD since it is known that both of them induce hepatosteatosis, with an increase in ROS and oxidative stress [18,44]. The gene discussed is SIRT3; the disease is metabolic dysfunction-associated steatotic liver disease.