Tumor cells within BMETs retained their luminal-type structure (Figure 2B inset, left) and ERα expression (Figure 2B inset, right, brown), and tumor cells isolated from these ER+ BMETs (43-4M from MCF-7-inoculated and 84-2MJ from MCF-7J-inoculated) retained the characteristics of inoculated cells with regards to TGFβ-inducible Smad signaling, albeit with somewhat reduced levels of Smad expression (Figure 1B); ER, TGFβRII, and TGFβRI receptor expression (Figure 2C); and epithelial phenotype (expressing E-cadherin, but not vimentin, contrasting with ER- MDA-SA cells, Supplemental Figure S2). This evidence concerns the gene ESR1 and neoplasm.