In the present study, we observed a significant effect of both colitis induction and beta-glucan supplementation on the gene expression level of pro-inflammatory cytokines, including Tnf, Il1a, Il1b, Il17a, and Il17b. A three-day supplementation with low molar mass oat beta-glucans reduced the expression of the Tnf gene. This evidence concerns the gene IL1B and colitis.