This situation prompted us to investigate the yield of EBUS-TBNA specimens, a cytological methodology, using a comprehensive multiplex genotyping based on combined next-generation sequencing (NGS) (DNA) and nCounter (RNA) multiplex testing [18], as well as PD-L1, in a series of patients with newly diagnosed NSCLC at our institution. The gene discussed is CD274; the disease is non-small cell lung carcinoma.