NGLY1 and Creutzfeldt Jacob disease: As shown previously [27], without PNGase F treatment, 5 PrP bands were detected by the V14 antibody in sCJD and non-CJD brain homogenates from the top to the bottom of the blot: Mono181, unglycosylated full-length PrP (FL), C-terminally truncated PrP fragment mono-glycosylated at residue 181 (Mono181-C1), C-terminally truncated PrP fragment 2 (C2), and C-terminally truncated PrP fragment 1 (C1) (Figure 5A) (Supplemental Table S1).