As shown in Figure 2d–g, tTau and p181-Tau were increased in the Ctx, Hp and NEVs of 3xTg-AD mice compared to WT, 2xTg-AD and 5xFAD mice (one-way ANOVA; tTau in Ctx: p < 0.0001 vs. WT and 2xTg-AD mice, p = 0.0019 vs. 5xFAD mice; tTau in Hp: p < 0.0001 vs. WT, 2xTg-AD and 5xFAD mice; tTau in NEVs: p = 0.0342 vs. WT mice; p181-Tau in Ctx: p < 0.0001 vs. WT and 5xFAD, p = 0.0102 vs. 2xTg-AD mice; p181-Tau in Hp: p < 0.0001 vs. WT and 5xFAD, p = 0.0021 vs. 2xTg-AD mice; p181-Tau in NEVs: p = 0.0004 vs. WT mice and p = 0.0009 vs. 5xFAD mice). Here, MAPT is linked to Alzheimer disease.