Human Tau species in the brain of 3xTg-AD mice increased from 6 to 8 to 10 months of age (Figure 2h,i; tTau in Ctx: p = 0.0313 for 8 vs. 10 months; tTau in Hp: p = 0.0028 for 6 vs. 10 months and p = 0.0082 for 8 vs. 10 months; one-way ANOVA), consistent with immunoblotting results (Figure 2b,c) and previous reports [34,38]. Here, HP is linked to Alzheimer disease.