Second, Western blot (WB) results from a representative group of 3xTg-AD mice (Figure 2b,c) showed an age-dependent accumulation of tTau and p181-Tau, in the Ctx and Hp of 3xTg-AD compared to WT mice (tTau in Ctx: p = 0.0103 at 10 months; in Hp: p = 0.0002 at 8 months and p = 0.0381 at 10 months; p181-Tau in Ctx: p = 0.0214 at 10 months; in Hp: p = 0.0077 at eight months and p = 0.0099 at 10 months; one-way ANOVA). This evidence concerns the gene HP and Alzheimer disease.