Interestingly, stretch-induced, TGF-β- or EGF-treated ex vivo CF isolated from mice with global TREK-1 KO, had a significant reduction in JNK and c-Jun phosphorylation when compared to CF isolated from WT mice, whereas ERK1/2 and p38 signalling was unaffected [234]. This evidence concerns the gene JUN and cystic fibrosis.