Since no mutations are known for p14ARF in LoVo cells, we verified the p14ARF and p16INK4a status by immunodetection, showing that among all CRC cell lines tested only LoVo express p14ARF protein, indicating LoVo cells to be p14ARF proficient (Figure 6C). The gene discussed is CDKN2A; the disease is colorectal carcinoma.