Importantly, VEGFA mRNA was detected in EVs from all eight cancer cell lines tested, including colorectal cancer cells (CACO-2, SW480, SW620), pancreatic cancer cells (MiaPaCa-2), lung cancer cells (H1650, H1792), gastric cancer cells (GC38) and liver cancer cells (HepG2) (Figure 2D). This evidence concerns the gene VEGFA and liver cancer.