Based on these considerations, in this study, we investigated the impact of relevant druggable DDR players on the survival of patient-derived CRC-SCs, identifying MRE11 homolog, double-strand break repair nuclease (MRE11) and RAD51 recombinase (RAD51) as targets for sensitizing CRC-SCs to CHK1 inhibitors. This evidence concerns the gene CHEK1 and colorectal carcinoma.