The ARPE-19 cell line used in this study was originally developed from the retinal pigment epithelium (RPE) of a human donor eye and resembles the phenotype and properties characteristic of aged native human RPE cells, lack of pigmentation, weak tight junctions, reduced expression of all-trans retinol, Pigment-Epithelium-Derived Factor (PEDF), and RPE markers, and hypersensitivity to VEGF activity, thereby making the ARPE-19 cell line an ideal in vitro AMD model [30]. This evidence concerns the gene VEGFA and age-related macular degeneration.