Therefore, we further validated the expression of Th1-associated chemokine genes, including Cxcl9, Cxcl10, and Ccl5, and Th2/Treg-associated chemokine genes, including Ccl17 and Ccl22, in carcinogen-induced tongue tumors using qRT-PCR. This evidence concerns the gene CXCL10 and tongue neoplasm.