HMOX1 and ischemia: One day after ischemia, HO-1 immunoreactivity was apparently expressed in glia-like cells located in all layers, and the immunoreactivity gradually increased with time after ischemia, showing that the ROD was 154.3% at 1 day, 179.0% at 2 days and 206.2% at 5 days after ischemia compared with that in the Normo + sham group (Figure 3Ad–f,C).