To evaluate the influence of TCDD, rapamycin, or combined TCDD and rapamycin on proinflammatory cytokines associated with the pathogenesis of psoriasis in vivo, TNF-α, IL-6, IL-17A, IL-22, and IL-23 mRNA expressions were assessed using qPCR in mouse skin tissue from five different treatment groups (control, IMQ, IMQ + TCDD, IMQ + rapamycin, or IMQ + TCDD + rapamycin) (Figure 7A–E). Here, TNF is linked to psoriasis.