The treatment of human RCC-derived (769-P and A498) and embryonic kidney cells (HEK293) with the demethylating agent 5-aza-2′-deoxycytidine resulted in a notable increase of PROX1-AS1 expression level (Figure 4) with the most significant change in HEK293 cells. This evidence concerns the gene PROX1 and renal cell carcinoma.