This method has been used to investigate a novel mechanism of NOTCH-induced tumor suppression involving modulation of the deacetylase SIRT1 [386]; to test the effects of the MDM2 inhibitor Nutlin-3 in combination with the small molecule YK-4-279 [387]; a role for receptor tyrosine kinase RON in EwS metastatic progression [388]; and to demonstrate synergistic effects of aurora kinase A inhibitors when combined with FAK inhibitors [389]. Here, SIRT1 is linked to neoplasm.