Similar to the immunohistochemistry results, the relative expression of Foxp3 was significantly higher in the STAT6−/− AOM/DSS mice, compared to the WT AOM/DSS mice, at Day 20 (15 ± 6.4 vs. 0.9 ± 0.07, p < 0.05) (Figure 2C). This evidence concerns the gene FOXP3 and infectious otitis media.