Therefore, we confirmed, in both human melanoma cell lines MUG Mel-1 and MUG Mel-2, the expressions of CD40L and ICOSL following infection with AdV-D24-ICOSL-CD40L by Western blotting (Figure 2C) and ELISA (Figure 3A,B) analysis after infection in A549 cells. This evidence concerns the gene ICOSLG and infection.