Puerarin suppressed invasion and vascularization of endometriosis tissue activated by E2 through reducing the accumulation of MMP-9, ICAM-1, and VEGF and increasing the level of tissue inhibitor of metalloproteinase 1 (TIMP-1) in primary endometriotic stromal cells and in chick chorioallantoic membrane in vivo model [100]. The gene discussed is TIMP1; the disease is endometriosis.