Figure 3B illustrates that inhibition of CD81 significantly enhanced the expression of radiation-induced γ-H2AX. Moreover, we visualized γ-H2AX foci by immunofluorescence assay and found that the nuclear γ-H2AX foci accumulation was also significantly increased in siCD81-transfected GBM cells in comparison with siNC group at 2 h after 6 Gy IR (Figure 3C). Here, H2AX is linked to glioblastoma.