Figure 7A illustrates that, at 30 min post-IR, Rad51 was mainly detected around the nuclear where CD81 located, and few Rad51 foci were formed inside of nuclear. However, at 2 h post-IR, a large amount of Rad51 foci were translocated from nuclear membrane to intra-nucleus. Furthermore, nuclear Rad51 foci in U251, U251R, and T98G cells were increased orderly, consistent with the CD81 levels in these cell lines. Therefore, Rad51 could translocate into nuclear in a time-dependent manner with the assistance of CD81 during HRR of GBM cells (Figure 7B). Here, CD81 is linked to glioblastoma.