We assessed expression of ROPN1B, NY-ESO-1 (single protein from CTAG1A or CTAG1B genes), MLANA and SOX10 (used here as a melanoma tumour marker) at the protein level by multispectral immunohistochemistry on melanoma tissue microarrays (TMAs) comprising two or three cores from 61 patient tumours (Table 1). The gene discussed is CTAG1A; the disease is melanoma.