By contrast, a semi-quantitative analysis of glomerulosclerosis using PAS-stained sections carried out by an observer blind to the treatment groups (age or genotype) revealed a partial but significant protective effect of TRPC6 inactivation revealed by two-way ANOVA for the interaction between age and genotype on glomerular score (Figure 5). The gene discussed is TRPC6; the disease is glomerulosclerosis.