A crossover study conducted in the U.S. [52] including 11 patients with T2D (mean age = 54 years) showed that mean PP glucose levels, iAUC for PP glucose, PP insulin levels and iAUC for insulin were significantly lower when non-starchy vegetables (lettuce and tomato salad with low-fat dressing and steamed broccoli with butter) and protein were consumed first, before carbohydrates, compared with the reverse food order. The gene discussed is INS; the disease is type 2 diabetes mellitus.